gi|296495135|ref|ADTO00000000.1| Escherichia coli MS 45-1 strain MS45-1 .1, whole .4994869, GC%: 50.41%, length = 4994869 bps

Total : 8 prophage regions have been identified, of which 3 regions are intact, 3 regions are incomplete, 2 regions are questionable.
REGIONREGION_LENGTHCOMPLETENESSSCORE#CDSREGION_POSITIONPOSSIBLE PHAGEGC_PERCENTAGEDETAIL
1 26.5Kb intact 100 33 288998-315531 Enterobacteria phage mEp460 50.06% Detail
2 18.3Kb incomplete 50 17 586521-604872 Enterobacteria phage N15 50.68% Detail
3 23.5Kb questionable 90 46 734496-758012 Enterobacteria phage mEp460 47.43% Detail
4 33.7Kb questionable 70 51 959777-993512 PHAGE_Stx2_c_II 47.61% Detail
5 10.5Kb intact 100 18 1768457-1778986 Enterobacteria phage HK629 54.52% Detail
6 4.2Kb incomplete 60 2201725-2205993 PHAGE_Gifsy_1 51.21% Detail
7 12.9Kb incomplete 50 18 2423780-2436768 Stx2-converting phage 1717 52.64% Detail
8 23.1Kb intact 150 24 3456069-3479193 Enterobacteria phage HK629 52.74% Detail
Legend:
REGION: the number assigned to the region
REGION_LENGTH: the length of the sequence of that region (in bp)
COMPLETENESS: a prediction of whether the region contains a intact or incomplete prophage based on the above criteria
SCORE: the score of the region based on the above criteria
#CDS: the number of coding sequnce
REGION_POSITION: the start and end positions of the region on the bacterial chromosome
PHAGE: the phage with the highest number of proteins most similar to those in the region
GC_PERCENTAGE: the percentage of gc nucleotides of the region
DETAIL: detail info of the region


txt file for download


Criteria for scoring prophage regions (as intact, questionable, or incomplete):
Method 1:
1. If the number of certain phage organism in this table is more than or equal to 100% of the total number of CDS of the region,
    the region is marked with total score 150. If less than 100%, method 2 and 3 will be used.

Method 2:
1. If the number of certain phage organism in this table is more than 50% of the total number of CDS of the region, that phage
    organism is considered as the major potential phage for that region; the percentage of the total number
    of that phage organism in this table in the total number of proteins of the region is calculated and
    then multipled by 100; the percentage of the length of that phage organism in this table in the length
    of the region is calculated and then multipled by 50 (phage head's encapsulation capability is considered).

Method 3:
1. If any of the specific phage-related keywords (such as 'capsid', 'head', 'integrase', 'plate', 'tail', 'fiber',
    'coat', 'transposase', 'portal', 'terminase', 'protease' or 'lysin') are present, the score will be increased
    by 10 for each keyword found.
2. If the size of the region is greater than 30 Kb, the score will be increased by 10.
3. If there are at least 40 proteins in the region, the score will be increased by 10.
4. If all of the phage-related proteins and hypothetical proteins constitute more than 70% of
    the total number of proteins in the region, the score will be increased by 10.

Compared the total score of method 2 with the total score of method 3, the bigger one is chosen as the total score of the region.
If the region's total score is less than 70, it is marked as incomplete; if between 70 to 90, it is marked as questionable; if greater than 90, it is marked as intact.