gi|330410604|ref|ACWP00000000.1| Lachnospiraceae bacterium 3_1_46FAA .1, whole genome shotgun .3092386, GC%: 42.03%, length = 3092386 bps

Total : 9 prophage regions have been identified, of which 1 regions are intact, 5 regions are incomplete, 3 regions are questionable.
REGIONREGION_LENGTHCOMPLETENESSSCORE#CDSREGION_POSITIONPOSSIBLE PHAGEGC_PERCENTAGEDETAIL
1 27.7Kb incomplete 50 15 2147-29921 Staphylococcus phage SMSAP5 38.16% Detail
2 30.6Kb questionable 80 28 1382770-1413438 Clostridium phage phiMMP04 43.24% Detail
3 35Kb incomplete 40 63 1418350-1453397 Clostridium phage phi8074-B1 41.76% Detail
4 33.5Kb questionable 70 38 2020755-2054340 Lactococcus phage 1706 40.54% Detail
5 11.8Kb incomplete 30 18 2650592-2662431 Lactococcus phage phiLC3 42.20% Detail
6 30.2Kb incomplete 30 25 2659323-2689611 Listeria phage B025 39.89% Detail
7 24.1Kb intact 110 22 2779278-2803405 Bacillus phage Fah 54.86% Detail
8 24.6Kb questionable 70 16 2798378-2822986 Bacillus phage phIS3501 46.55% Detail
9 17.5Kb incomplete 10 23 2836329-2853846 Staphylococcus phage phi7401PVL 44.67% Detail
Legend:
REGION: the number assigned to the region
REGION_LENGTH: the length of the sequence of that region (in bp)
COMPLETENESS: a prediction of whether the region contains a intact or incomplete prophage based on the above criteria
SCORE: the score of the region based on the above criteria
#CDS: the number of coding sequnce
REGION_POSITION: the start and end positions of the region on the bacterial chromosome
PHAGE: the phage with the highest number of proteins most similar to those in the region
GC_PERCENTAGE: the percentage of gc nucleotides of the region
DETAIL: detail info of the region


txt file for download


Criteria for scoring prophage regions (as intact, questionable, or incomplete):
Method 1:
1. If the number of certain phage organism in this table is more than or equal to 100% of the total number of CDS of the region,
    the region is marked with total score 150. If less than 100%, method 2 and 3 will be used.

Method 2:
1. If the number of certain phage organism in this table is more than 50% of the total number of CDS of the region, that phage
    organism is considered as the major potential phage for that region; the percentage of the total number
    of that phage organism in this table in the total number of proteins of the region is calculated and
    then multipled by 100; the percentage of the length of that phage organism in this table in the length
    of the region is calculated and then multipled by 50 (phage head's encapsulation capability is considered).

Method 3:
1. If any of the specific phage-related keywords (such as 'capsid', 'head', 'integrase', 'plate', 'tail', 'fiber',
    'coat', 'transposase', 'portal', 'terminase', 'protease' or 'lysin') are present, the score will be increased
    by 10 for each keyword found.
2. If the size of the region is greater than 30 Kb, the score will be increased by 10.
3. If there are at least 40 proteins in the region, the score will be increased by 10.
4. If all of the phage-related proteins and hypothetical proteins constitute more than 70% of
    the total number of proteins in the region, the score will be increased by 10.

Compared the total score of method 2 with the total score of method 3, the bigger one is chosen as the total score of the region.
If the region's total score is less than 70, it is marked as incomplete; if between 70 to 90, it is marked as questionable; if greater than 90, it is marked as intact.