gi|228860918|ref|ACNL00000000.1| Bacillus thuringiensis IBL 4222 , whole genome shotgun .6574132, GC%: 34.85%, length = 6574132 bps

Total : 9 prophage regions have been identified, of which 2 regions are intact, 5 regions are incomplete, 2 regions are questionable.
REGIONREGION_LENGTHCOMPLETENESSSCORE#CDSREGION_POSITIONPOSSIBLE PHAGEGC_PERCENTAGEDETAIL
1 30.5Kb questionable 70 25 369536-400068 Bacillus phage phIS3501 35.01% Detail
2 29.3Kb incomplete 10 26 3118961-3148323 Bacillus phage BtCS33 36.81% Detail
3 28.5Kb incomplete 30 14 5421136-5449642 Clostridium phage phiCD27 33.10% Detail
4 23.5Kb questionable 90 26 5446583-5470115 Bacillus phage phIS3501 35.01% Detail
5 12.4Kb incomplete 30 21 5494015-5506509 Bacillus phage phIS3501 33.43% Detail
6 14.2Kb incomplete 10 16 5594222-5608449 PHAGE_Bacill_36 36.09% Detail
7 33.4Kb incomplete 50 48 5625877-5659363 PHAGE_Bacill_SPBc2 36.18% Detail
8 45.4Kb intact 110 39 5953734-5999161 Bacillus virus 1 34.98% Detail
9 14.4Kb intact 117 25 6515847-6530309 Bacillus phage Bam35c 40.01% Detail
Legend:
REGION: the number assigned to the region
REGION_LENGTH: the length of the sequence of that region (in bp)
COMPLETENESS: a prediction of whether the region contains a intact or incomplete prophage based on the above criteria
SCORE: the score of the region based on the above criteria
#CDS: the number of coding sequnce
REGION_POSITION: the start and end positions of the region on the bacterial chromosome
PHAGE: the phage with the highest number of proteins most similar to those in the region
GC_PERCENTAGE: the percentage of gc nucleotides of the region
DETAIL: detail info of the region


txt file for download


Criteria for scoring prophage regions (as intact, questionable, or incomplete):
Method 1:
1. If the number of certain phage organism in this table is more than or equal to 100% of the total number of CDS of the region,
    the region is marked with total score 150. If less than 100%, method 2 and 3 will be used.

Method 2:
1. If the number of certain phage organism in this table is more than 50% of the total number of CDS of the region, that phage
    organism is considered as the major potential phage for that region; the percentage of the total number
    of that phage organism in this table in the total number of proteins of the region is calculated and
    then multipled by 100; the percentage of the length of that phage organism in this table in the length
    of the region is calculated and then multipled by 50 (phage head's encapsulation capability is considered).

Method 3:
1. If any of the specific phage-related keywords (such as 'capsid', 'head', 'integrase', 'plate', 'tail', 'fiber',
    'coat', 'transposase', 'portal', 'terminase', 'protease' or 'lysin') are present, the score will be increased
    by 10 for each keyword found.
2. If the size of the region is greater than 30 Kb, the score will be increased by 10.
3. If there are at least 40 proteins in the region, the score will be increased by 10.
4. If all of the phage-related proteins and hypothetical proteins constitute more than 70% of
    the total number of proteins in the region, the score will be increased by 10.

Compared the total score of method 2 with the total score of method 3, the bigger one is chosen as the total score of the region.
If the region's total score is less than 70, it is marked as incomplete; if between 70 to 90, it is marked as questionable; if greater than 90, it is marked as intact.