gi|190904988|ref|AAJT00000000.2| Escherichia coli B7A , whole genome shotgun .5300242, GC%: 50.68%, length = 5300242 bps

Total : 9 prophage regions have been identified, of which 4 regions are intact, 3 regions are incomplete, 2 regions are questionable.
REGIONREGION_LENGTHCOMPLETENESSSCORE#CDSREGION_POSITIONPOSSIBLE PHAGEGC_PERCENTAGEDETAIL
1 15.3Kb incomplete 30 10 113-15435 Enterobacteria phage lambda 47.95% Detail
2 43.8Kb intact 120 52 612037-655912 Enterobacteria phage IME10 47.09% Detail
3 44.8Kb intact 150 61 1764682-1809515 Enterobacteria phage lambda 50.38% Detail
4 25.7Kb incomplete 10 38 3566101-3591881 Burkholderia phage BcepMu 54.06% Detail
5 19.4Kb intact 100 26 3643929-3663408 Salmonella phage ST64B 53.35% Detail
6 49.6Kb intact 110 36 4045221-4094907 PHAGE_Yersin_413C 49.12% Detail
7 21.5Kb questionable 80 27 4442784-4464352 Enterobacteria phage phiP27 53.38% Detail
8 11.8Kb questionable 80 15 4731104-4742965 Burkholderia phage phiE255 52.27% Detail
9 5.5Kb incomplete 50 4899306-4904819 Enterobacteria phage mEp460 52.63% Detail
Legend:
REGION: the number assigned to the region
REGION_LENGTH: the length of the sequence of that region (in bp)
COMPLETENESS: a prediction of whether the region contains a intact or incomplete prophage based on the above criteria
SCORE: the score of the region based on the above criteria
#CDS: the number of coding sequnce
REGION_POSITION: the start and end positions of the region on the bacterial chromosome
PHAGE: the phage with the highest number of proteins most similar to those in the region
GC_PERCENTAGE: the percentage of gc nucleotides of the region
DETAIL: detail info of the region


txt file for download


Criteria for scoring prophage regions (as intact, questionable, or incomplete):
Method 1:
1. If the number of certain phage organism in this table is more than or equal to 100% of the total number of CDS of the region,
    the region is marked with total score 150. If less than 100%, method 2 and 3 will be used.

Method 2:
1. If the number of certain phage organism in this table is more than 50% of the total number of CDS of the region, that phage
    organism is considered as the major potential phage for that region; the percentage of the total number
    of that phage organism in this table in the total number of proteins of the region is calculated and
    then multipled by 100; the percentage of the length of that phage organism in this table in the length
    of the region is calculated and then multipled by 50 (phage head's encapsulation capability is considered).

Method 3:
1. If any of the specific phage-related keywords (such as 'capsid', 'head', 'integrase', 'plate', 'tail', 'fiber',
    'coat', 'transposase', 'portal', 'terminase', 'protease' or 'lysin') are present, the score will be increased
    by 10 for each keyword found.
2. If the size of the region is greater than 30 Kb, the score will be increased by 10.
3. If there are at least 40 proteins in the region, the score will be increased by 10.
4. If all of the phage-related proteins and hypothetical proteins constitute more than 70% of
    the total number of proteins in the region, the score will be increased by 10.

Compared the total score of method 2 with the total score of method 3, the bigger one is chosen as the total score of the region.
If the region's total score is less than 70, it is marked as incomplete; if between 70 to 90, it is marked as questionable; if greater than 90, it is marked as intact.